The function and the intracellular localization of <I>Arabidopsis</I> COP1
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- Mochiduki Kumi
- Grad. School of Human. and Sci., Ochanomizu Univ.
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- Deng Xing-Wang
- Dept of Mol., Cell., and Develop. Biol., Yale Univ.
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- Yamamoto Naoki
- Dept. of Sci., Ochanomizu Univ.
Bibliographic Information
- Other Title
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- シロイヌナズナCOP1の細胞内分布特性と機能発現
Abstract
Photomorphogenic repressor COP1 of Arabidopsis has been shown to repress the activity of transcription factors in the nucleus in the dark. However, light has diminished the nuclear localization of COP1 and abrogate the repressive activities. To modify the intracellular localization of COP1, we introduced mutation in the NLS and fused to GFP gene [GFP-COP1 (Mut)]. In a transient assay in onion epidermal cells, GFP-COP1 was localized to the nucleus but GFP-COP1 (Mut) was localized to cytoplasm. The chimeric genes were introduced into Arabidopsis. In the stable transformants of GFP-COP1 (Mut), opening and expansion of cotyledons were observed but apical hook was not even in complete darkness. However, the GFP-COP1 plants did show the normal skotomorphogenesis and photomorphogenesis in the dark and light, respectively. These suggest that GFP-COP1 (Mut) affects the development of seedlings in the dark. Here, we discuss the relationship between the function and the intracellular localization of COP1.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2003 (0), 226-226, 2003
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680605774464
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- NII Article ID
- 130006989456
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed