Functional analysis of a T7 phage-type RNA polymerase RpoT;2 in <I>Arabidopsis</I>
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- Kanamaru Kengo
- Lab. of Biochem., Fac. of Agr., Kobe Univ.
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- Ichibayashi Hisao
- Lab. of Biochem.,Grad. School of Sci. & Tech., Kobe Univ.
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- Inagaki Ryosaku
- Lab. of Biochem.,Grad. School of Sci. & Tech., Kobe Univ.
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- Ito Shigekazu
- Lab. of Biochem.,Grad. School of Sci. & Tech., Kobe Univ.
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- Kanechi Michio
- Lab. of Veget. & Ornam. Plant Sci., Fac. of Agr., Kobe Univ.
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- Sakurai Nozomu
- 2nd lab for Plant Gen. Res., Kazusa DNA Res. Inst.
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- Suzuki Hideyuki
- 2nd lab for Plant Gen. Res., Kazusa DNA Res. Inst.
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- Shibata Daisuke
- 2nd lab for Plant Gen. Res., Kazusa DNA Res. Inst.
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- Uno Tomohide
- Lab. of Biochem., Fac. of Agr., Kobe Univ.
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- Yamagata Hiroshi
- Lab. of Biochem., Fac. of Agr., Kobe Univ.
Bibliographic Information
- Other Title
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- シロイヌナズナT7ファージ型RNAポリメラーゼRpoT;2の機能解析
Description
Plant has three genomes in nucleus, mitochondria, and plastids, respectively. Gene expressions from these genomes are seemed to coordinated with each other through an undefined crosstalk mechanism. They are proceeded by T7 phage-type RNA polymerase (NEP) in mitochondria, whereas by NEP and eubacteria-type RNA polymerase (PEP) in chloroplasts. Arabidopsis has three different NEPs, namely, mitochondria-localized RpoT;1(RpoTm), chloroplast-localized RpoT;3(RpoTp), and possible dual-localized RpoT;2(RpoTmp). In this study, we addressed if the RpoT;2 had critical roles in transcription of mitochondria and/or chloroplast genome(s), through the analysis of a RpoT;2 knockout mutant. Although Baba et al. has already reported about an allele of the RpoT;2 mutant in 2004, the definite function of RpoT;2 is still unclear. We performed northern analysis, QPCR analysis, microarray analysis, measurement of photosynthesis activity and so on. We will show the data and suggest critical roles of RpoT;2 in some gene expression.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 179-179, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680605938816
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- NII Article ID
- 130006989643
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed