Functional analysis of a T7 phage-type RNA polymerase RpoT;2 in <I>Arabidopsis</I>

DOI

Bibliographic Information

Other Title
  • シロイヌナズナT7ファージ型RNAポリメラーゼRpoT;2の機能解析

Description

Plant has three genomes in nucleus, mitochondria, and plastids, respectively. Gene expressions from these genomes are seemed to coordinated with each other through an undefined crosstalk mechanism. They are proceeded by T7 phage-type RNA polymerase (NEP) in mitochondria, whereas by NEP and eubacteria-type RNA polymerase (PEP) in chloroplasts. Arabidopsis has three different NEPs, namely, mitochondria-localized RpoT;1(RpoTm), chloroplast-localized RpoT;3(RpoTp), and possible dual-localized RpoT;2(RpoTmp). In this study, we addressed if the RpoT;2 had critical roles in transcription of mitochondria and/or chloroplast genome(s), through the analysis of a RpoT;2 knockout mutant. Although Baba et al. has already reported about an allele of the RpoT;2 mutant in 2004, the definite function of RpoT;2 is still unclear. We performed northern analysis, QPCR analysis, microarray analysis, measurement of photosynthesis activity and so on. We will show the data and suggest critical roles of RpoT;2 in some gene expression.

Journal

Details 詳細情報について

  • CRID
    1390282680605938816
  • NII Article ID
    130006989643
  • DOI
    10.14841/jspp.2006.0.179.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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