ARC3, Chloroplast Division Factor, Is A Prokaryotic And Eukaryotic Fusion Protein

DOI
  • Shimada Hiroshi
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Koizumi Masato
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Mochizuki Mariko
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Fujimoto Hiotshi
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Kuroki Kouta
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Masuda Tatsuru
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Ohta Hiroyuki
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
  • Takamiya Ken-ichiro
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

Bibliographic Information

Other Title
  • 葉緑体分裂制御因子ARC3は原核生物分裂装置と真核生物シグナル伝達因子の融合タンパク質である

Description

It is widely believed that chloroplast was generated by endosymbiosis of ancient photosynthetic bacterium into ancient eukaryotic cell. arc3 (Accumulation and Replication of Chloroplast) mutant of Arabidopsis thaliana has a few abnormally large chloroplasts in cell. Fine mapping analyses showed ARC3 gene is a fusion gene of prokaryotic, FtsZ, and eukaryotic, phosphatidylinositol-4-phosphate 5-kinase (PIP5K), genes. The fusion protein of the full-length ARC3 and GFP (Green fluorescent protein) was located exclusively in chloroplast. The PIP5K activity of ARC3 protein was not detected. The ARC3 protein, however is phosphorylated by A. thaliana protein in vitro. These results show that ARC3 is a functional prokaryotic and eukaryotic fusion protein, and is a linkage point in the signal transduction by which eukaryotic nucleus regulates prokaryotic chloroplast division.

Journal

Details 詳細情報について

  • CRID
    1390282680606143872
  • NII Article ID
    130006989884
  • DOI
    10.14841/jspp.2003.0.319.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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