Characterization of Dark-Inducible Polyphenol Oxidase-Like Protein in <I>Lithospermum erythrorhison</I>
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- Yamamoto Kyoko
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Mito Koji
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Sasaki Kanako
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Ohara Kazuaki
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
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- Sakurai Nozomu
- Kazusa DNA Research Institute
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Yazaki Kazufumi
- Research Institute for Sustainable Humanosphere, Kyoto Univ.
Bibliographic Information
- Other Title
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- 暗黒下特異的に発現するPolyphenol Oxidase-Like Proteinの機能解析
Abstract
Lithospermum erythrorhizon produces red naphthoquinone pigments called shikonin derivatives in the roots. In the cell cultures of L. erythrorhizon, stimulators of shikonin biosynthesis (Cu2+ and MJ) as well as its inhibitors (NH4+ and light) have been identified. Shikonin is biosynthesized from geranyldiphosphate derived via mevalonate pathway and p-hydroxybenzoate via shikimate pathway, whereas the key step of naphthalene ring formation is not clarified yet. <br>To isolate cDNAs specifically expressed under shikonin production, we applied PCR-select cDNA subtraction technique, where dark-inducible genes were enriched. Out of 240 clones whose expressions were enhanced in the dark, we have selected a polyphenol oxidase-like protein as a candidate gene for the naphthalene-forming enzyme. Time course experiment of the northern blot showed that the gene expression completely paralleled the shikonin production pattern by various regulatory factors. We are preparing the recombinant enzyme to characterize its catalytic function.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 066-066, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680606646400
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- NII Article ID
- 130006990398
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed