Function of Nucleotide Pyrophosphatase/Phosphodiesterase Gene Family in Rice -Analysis of Intracellular Localization
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- Kaneko Kentarou
- Grad. Sch. Sci. Tech. Niigata Univ
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- Yamada Chie
- Grad. Sch. Sci. Tech. Niigata Univ
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- Yanagida Ai
- Dept. Applied Biol. Chem. Niigata Univ
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- Kitajima Aya
- Grad. Sch. Sci. Tech. Niigata Univ
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- Itou Kimiko
- Grad. Sch. Sci. Tech. Niigata Univ
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- Mitsui Toshiaki
- Grad. Sch. Sci. Tech. Niigata Univ
Bibliographic Information
- Other Title
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- イネ<i>NPP</I>遺伝子ファミリーの機能解析 -NPP1,2及び6は葉緑体に局在する-
Abstract
We investigate function of nucleotide pyrophosphatase/phosphodiesterase (NPP) gene family in rice (Oryza sativa L.). Six genes of NPP homologues are existed and located on chromosomes 3 (NPP3), 8 (NPP1), 9 (NPP6) and 12 (NPP2, 4, 5). Recombinant proteins for NPP1, 2 and 6 were expressed and purified from transgenic rice cells. Computer-assisted analyses showed that the deduced amino-acid sequences contain numerous N-glycosylation sites and cleavable hydrophobic signal sequences. All NPP1, 2 and 6 proteins actually conatined N-linked oligosaccharide chains recognized by Con A and Endo-H. However, confocal laser scanning microscopy of rice cells expressing NPP1-GFP, NPP2-GFP and NPP6-GFP revealed that they similarly localize in the chroloplast compartment. The results obtained suggest that the NPP family is synthesized and transported from ER-Golgi system to plastids, and possibly involved in the control of plastidial levels of nucleotides linked to glycosylation.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2008 (0), 0502-0502, 2008
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680606670080
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- NII Article ID
- 130006990436
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed