Identification of the novel peptide ligands involved in plant organ deveropment

DOI

Bibliographic Information

Other Title
  • 植物の器官形成に関与する新奇ペプチド性シグナル分子の同定

Abstract

In organ development the intercellular communications occur between shoot apical meristem (SAM) and lateral organ primordium. Recently several peptide ligands responsible for SAM organization or lateral organ development are reported, but it is not enough to understand all developmental events there. And it is indispensable to identify novel peptide ligands from SAM. Therefore we tried to identify peptide ligand from extracellular space of SAM. As a starting material, cauliflower or Arabidopsis ap1 cal mutant were used for identification. In cauliflower, proteins of 3000-18000 Da were analyzed by 2D gel electrophoresis following protein sequence, and using this method we identified 41 proteins. In Arabidopsis ap1 cal mutant, proteins of 500-4000 Da were separated by column chromatography and amino acid sequence was detected by LC-MS/MS. Here we identified 339 proteins categorized based on Arabidopsis database and selected 30 ligand candidates. We tested peptide activity by adding to Arabidopsis seedling and found abnormal structure in SAM and root.

Journal

Details 詳細情報について

  • CRID
    1390282680606851968
  • NII Article ID
    130006990709
  • DOI
    10.14841/jspp.2010.0.0531.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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