Identification of the novel peptide ligands involved in plant organ deveropment
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- Tsuchida Yuhei
- National Institute for Basic Biology
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- Igarashi Hisako
- National Institute for Basic Biology
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- Yabe Kimihiko
- National Institute for Basic Biology
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- Nakamori Chihiro
- National Institute for Basic Biology
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- Okada Kiyotaka
- National Institute for Basic Biology
Bibliographic Information
- Other Title
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- 植物の器官形成に関与する新奇ペプチド性シグナル分子の同定
Abstract
In organ development the intercellular communications occur between shoot apical meristem (SAM) and lateral organ primordium. Recently several peptide ligands responsible for SAM organization or lateral organ development are reported, but it is not enough to understand all developmental events there. And it is indispensable to identify novel peptide ligands from SAM. Therefore we tried to identify peptide ligand from extracellular space of SAM. As a starting material, cauliflower or Arabidopsis ap1 cal mutant were used for identification. In cauliflower, proteins of 3000-18000 Da were analyzed by 2D gel electrophoresis following protein sequence, and using this method we identified 41 proteins. In Arabidopsis ap1 cal mutant, proteins of 500-4000 Da were separated by column chromatography and amino acid sequence was detected by LC-MS/MS. Here we identified 339 proteins categorized based on Arabidopsis database and selected 30 ligand candidates. We tested peptide activity by adding to Arabidopsis seedling and found abnormal structure in SAM and root.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2010 (0), 0531-0531, 2010
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680606851968
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- NII Article ID
- 130006990709
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed