High-Throughput Screen for Enzyme Reactions in the Cinnamate-Monolignol Pathway through FT-ICR/MS Metabolomics
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- Kai Kosuke
- Research Association for Biotechnology
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- Tatsumi Mai
- Graduate School of Life and Environmental Sciences, Osaka Prefecture Univ.
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- Shibata Daisuke
- Kazusa DNA Res. Inst.
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- Kanaya Shigehiko
- Department of Bioinformatics and Genomics, NAIST
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- Ohta Daisaku
- Graduate School of Life and Environmental Sciences, Osaka Prefecture Univ.
Bibliographic Information
- Other Title
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- FT-ICR MS メタボロミクスによる桂皮酸モノリグノール経路の多段階酵素反応の同時解析
Description
The cinnamate-monolignol pathway, a biosynthetic route with one of the highest metabolic flows in plants, produces a wide variety of phenylpropanoid compounds. The pathway enzymes are often encoded by multiple genes and accept several substrates leading to the formation of a complex metabolic grid. A major challenge is due to the difficulties in understanding whether or not dynamic metabolic flows and physiological substrate combinations may be deducible through in vitro enzyme studies with putative substrate compounds. The extremely high sensitivity and resolution of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR/MS) warrant accurate MS measurements, and its performance has been developed as a unique metabolomics tool. Here, using the FT-ICR/MS metabolomics approach, we simultaneously studied sequential reactions using recombinant proteins of 4CL (4-coumarate:CoA ligase), HCT (hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyltransferase), and a cytochrome P450 with multiple substrates and discuss the potential of the FT-ICR/MS metabolomics for high-throughput screens for enzyme reactions.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 208-208, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680607118848
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- NII Article ID
- 130006991108
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed