Site-specific expression and subcellular localization of a signal peptide peptidase in <I>Arabidopsis thaliana</I>

DOI

Bibliographic Information

Other Title
  • シロイヌナズナのシグナルペプチドペプチダーゼの組織特異的発現および細胞内局在

Abstract

Signal peptide peptidase (SPP) was identified as a homologue of presenilin, catalyzes intramembrane proteolysis of some signal peptides cleaved from a preprotein. Searching the Arabidopsis database, we found an orthologue of human SPP (AtSPP) and five homologues (AtSPPL1-AtSPPL5). In situ hybridization showed that AtSPPs were highly expressed in the shoot meristem and the epidermis of radicle during germination. At the reproductive stage in shoot apex, AtSPP was expressed in the inflorescence meristem, whearas the expression of homologues took place in the lower part of rib zone, where cell division is activated to form vascular bundle. Subcellular localization of AtSPPs were investigated by transporting a GFP fused protein in Deep cells. We detected GFP-AtSPP on the ER membrane and both GFP-AtSPPL2 and GFP-AtSPPL3 in the endosomes. These results suggest that AtSPP and AtSPPLs are functionally different from one another, having differential roles in plant physiology.

Journal

Details 詳細情報について

  • CRID
    1390282680607347456
  • NII Article ID
    130006991439
  • DOI
    10.14841/jspp.2007.0.807.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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