Development of new methods to identify transcription factors that interact with promoter region
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- Mitsuda Nobutaka
- AIST
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- Kondou Youichi
- RIKEN PSC
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- Matsui Minami
- RIKEN PSC
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- Ohme-Takagi Masaru
- AIST
Bibliographic Information
- Other Title
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- 上流転写制御因子を同定する新技術の開発
Abstract
To identify transcription factors (TFs) that would interact with 5' upstream region of a gene, yeast one-hybrid screening (Y1H) is often used. For Y1H, we need to identify cis-regulatory region and multimerize it for placing them in upstream of reporter gene. In addition, large-scale screening is required because non-TF genes mostly occupy cDNA library. To overcome such problems, we constructed novel cDNA library that only contains TF genes. Results of our test experiments showed that several transcription factors were identified with efficiency more than 100-fold even though whole 500-1000bp promoter region was directly used as a probe. However, false-positive or false-negative result was sometimes observed in the yeast experimental system. Therefore we are now trying to develop new method using plant instead of yeast. We will report the results of comparison of these methods to identify upstream transcription factors.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2010 (0), 0167-0167, 2010
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680607715840
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- NII Article ID
- 130006991988
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed