Cell biological analysis of vacuole formation in plant cells

DOI

Bibliographic Information

Other Title
  • 植物細胞における液胞形成機構の細胞生物学的解析

Description

Vacuoles play many important roles in plant cells. In the root meristematic tissue, the presence of mature vacuoles is less evident, but they are always present as small provacuoles. It is known that provacuoles are produced by the trans Golgi network (TGN). When a cell begins to mature, the provacuoles fuse to produce large central vacuoles that are characteristic of most mature plant cells. We tried to prepare ultrathin sections of various plant samples by high-pressure freezing method and immunogold staining. In the root meristematic cells of Arabidopsis spp. and tobacco, vacuolar H+-pyrophosphatase (V-PPase) signals were detected in the TGN, tonoplast, and certain ring-like structures. These ring-like structures existed as flat and/or double-membrane structures and contained cellular components. Moreover, the structures fused with the central vacuoles. Therefore, these ring-like structures containing V-PPase may play an important role in vacuole formation and degradation of cellular components.

Journal

Details 詳細情報について

  • CRID
    1390282680607864576
  • NII Article ID
    130006992208
  • DOI
    10.14841/jspp.2010.0.0838.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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