Subcellular localization of plant acetylcholinesterase in transgenic rice

DOI

Bibliographic Information

Other Title
  • 植物アセチルコリンエステラーゼの細胞内局在性

Abstract

We previously detected that plant acetylcholinesterase (AChE) was in the cortex of coleoptile node of maize seedling by histochemical and DTNB method. We also identified AChE genes from maize and siratro seedlings. In this report, subcellular localization of the plant AChEs (maize AChE, rice AChE1 and rice AChE2) in leaf tissues of the transgenic rice plants was investigated. Two rice AChE genes were identified high AChE homologous genes based on our cloned maize AChE gene. Then, maize AChE and two rice AChEs were introduced rice plants, respectively. Localization of these AChEs was investigated by immunofluorescence in leaf tissues using antibody against maize AChE. The maize AChE and two rice AChEs were localized in extracellular spaces in the leaf of the plants. The results suggest that acetylcholine (ACh), ACh receptor (AChR) and AChE (ACh-mediated system) may function at extracellular region. Now we continued investigate localization of green fluorescent protein (GFP) fusion AChE in transgenic rice. This research was supported by grant from Japan Space Forum.

Journal

Details 詳細情報について

  • CRID
    1390282680607947392
  • NII Article ID
    130006992339
  • DOI
    10.14841/jspp.2010.0.0881.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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