Molecular cloning and characterization of rosmarinic acid synthase from <I>Lithospermum erythrorhizon</I>
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- Yamashita Hiromi
- Graduate school of Pharmaceutical Sciences, Nagoya City University
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- Terasaka Kazuyoshi
- Graduate school of Pharmaceutical Sciences, Nagoya City University
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- Mito Koji
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Sakurai Nozomu
- Kazusa DNA Research Institute
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Yazaki Kazufumi
- Research Institute for Sustainable Humanosphere, Kyoto University
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- Mizukami Hajime
- Graduate school of Pharmaceutical Sciences, Nagoya City University
Bibliographic Information
- Other Title
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- ムラサキ培養細胞由来rosmarinic acid synthaseの単離と機能解析
Abstract
Lithospermum erythrorhizon produces rosmarinic acid (RA), a well-known hydroxycinnamic acid ester. In RA biosynthetic pathway, condensation of 4-coumaroyl CoA derived from phenylalanine, with 4-hydroxyphenyllactic acid (HPL) derived from tyrosine, results in formation of 4-coumaroyl-4'-hydroxyphenyllactic acid (CHPL) catalyzed by rosmarinic acid synthase (RAS). We attempted to isolate a cDNA of RAS from L. erythrorhizon. <br>To isolate cDNA, we screened cDNA library using informatics analysis of EST data. Four cDNA isolated were encoding acyltransferases (LeHCT1~4) with typical characteristics of the BAHD superfamily. We prepared the recombinant enzyme to characterize its catalytic function. LeHCT1 displayed the activity of RAS using 4-coumaroyl- and caffeoyl CoA and HPL as substrates. Shikimic acid was not able to serve as acyl acceptors. In L. erythrorhizon cell cultures, RA production is rapidly stimulated by addition methyl jasmonate (MJ), but RA activity is not induced. LeHCT1 mRNA was not induced in accordance with this pattern.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2008 (0), 0778-0778, 2008
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680608185216
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- NII Article ID
- 130006992696
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed