Functional analysis of extracellular components using immunomodulation.
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- Shinohara Naoki
- Graduate School of Science, The University of Tokyo
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- Fujita Miki
- University of British Columbia
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- Fukuda Hiroo
- Graduate School of Science, The University of Tokyo
Bibliographic Information
- Other Title
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- 免疫攪乱による細胞外分泌因子の機能解析
Abstract
To investigate phenotypes of a plant that expresses a monoclonal antibody is potentially useful to know in situ functions of its antigen. This method is called immunomodulation. It has been reported that plants expressing monoclonal antibodies against phytohormones show phenotypes similar to those of plants short in the phytohoromones. <br> We had isolated approximately one hundred of monoclonal antibodies that recognize molecules secreted from differentiating xylem cells by phage display subtraction. In this study, to find extracellular components that have important roles in xylem development, we introduced genes for the isolated antibodies into Arabidopsis plants for immunomodulation. The following three points were devised: 1) a promoter for antibody expression, 2) signals directing intracellular localization of antibodies, 3) negative control for evaluation of the causal linkage between phenotypes and antigen-antibody binding. We will report the results of the experiments.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 675-675, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680608433280
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- NII Article ID
- 130006993057
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed