Identification of an aquaporin involved in arsenite transport and tolerance.
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- Kamiya Takehiro
- Biotechnology Research Center, The University of Tokyo
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- Tanaka Mayuki
- Biotechnology Research Center, The University of Tokyo
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- Mitani Namiki
- Research Institute for Bioresoures, Okayama University
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- Ma Jian Feng
- Research Institute for Bioresoures, Okayama University
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- Maeshima Masayoshi
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Fujiwara Toru
- Biotechnology Research Center, The University of Tokyo SORST, JST
Bibliographic Information
- Other Title
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- 亜ヒ酸の輸送と耐性に関与するアクアポリン、NIP1;1
Abstract
To identify molecule(s) which mediates As(III) uptake in plants, we screened for As(III) tolerant mutants expecting that loss-of-function mutant of As(III) transporter would be tolerant to As(III). After screening of EMS-mutagenized Arabidopsis thaliana M2 seeds, we identified 3 independent mutants. We also analyzed tolerance of T-DNA mutants of NIPs, which are homologues of aquaglyceroporins. Among the lines tested, nip1;1 mutant showed As(III) tolerance. Sequencing of the NIP1;1 in the mutants revealed that each mutants had an independent mutation in NIP1;1, establishing that NIP1;1 is the causal gene of As(III) tolerance. Xenopus oocytes injected with NIP1;1 cRNA accumulated much As(III) than control oocytes injected with water. The promoter-GUS and real-time PCR analysis showed that NIP1;1 was highly expressed in roots. From these data, we conclude that NIP1;1 is the major As(III) transporter involved in As(III) uptake in roots in A. thaliana.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2009 (0), S0033-S0033, 2009
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680608470272
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- NII Article ID
- 130006993113
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed