Functional Analysis of Phytochrome in <I>Marchantia polymorpha</I>

DOI

Bibliographic Information

Other Title
  • 苔類ゼニゴケをモデルとしたフィトクロムシグナル伝達の解析

Abstract

To better understand the functions of phytochrome, we used the liverwort Marchantia polymorpha, which is haploid and has a simple body structure, as a unique model plant. We isolated a phytochrome gene in M. polymorpha, MpPHY. Southern-blot analysis suggested that MpPHY is a single-copy gene, whereas higher plants carry two or more copies of phytochrome gene. Mpphy(N612) showed Red/Far-Red photoreversibility typical of functional phytochrome in vitro. A single amino acid substitution mutant of Mpphy(N612), MpphyY241H(N612), exhibited no photoreversibility. In transgenic plants expressing MpphyY241H, formation of rhizoid was promoted regardless of light condition, and the blue light response was supressed. The MpphyY241H-GFP fusion protein localized in the nucleus forming speckles. These results indicate that MpphyY241H functions as a constitutively active form in M. polymorpha, and Mpphy controls photomorphogeneses.

Journal

Details 詳細情報について

  • CRID
    1390282680608486528
  • NII Article ID
    130006993138
  • DOI
    10.14841/jspp.2008.0.0093.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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