Functional Analysis for Circadian Clock-Related Genes of Duckweeds by Using a Transient Expression System

DOI
  • Serikawa Masayuki
    Division of Biological Science, Graduate School fo Science, Nagoya Univ.
  • Miwa Kumiko
    Division of Biological Science, Graduate School fo Science, Nagoya Univ.
  • Suzuki Sayaka
    Division of Biological Science, Graduate School fo Science, Nagoya Univ.
  • Kondo Takao
    Division of Biological Science, Graduate School fo Science, Nagoya Univ.
  • Oyama Tokitaka
    Division of Biological Science, Graduate School fo Science, Nagoya Univ.

Bibliographic Information

Other Title
  • 一過的発現系を用いたウキクサ概日時計関連遺伝子の機能解析

Abstract

The Lemna genus in duckweeds includes two species that show photoperiodic flowering responses of a long-day type (L. gibba) and a short-day type (L. paucicostata). We tried to reveal the molecular basis for their timing systems. We isolated a number of Lemna homologues of circadian clock-related genes of Arabidopsis. We then tried establishing the methods for analysis of functions of those homologues in the Lemna plants. To monitor the circadian rhythm, we developed a bioluminescent reporter system in which the luciferase gene under a circadian promoter was introduced by using a particle bombardment method. Then, this semi-transient bioluminescence monitoring system was combined with overexpression/RNAi procedures to manipulate the expression levels of clock-related homologues. This enabled us to try various combinations of effectors/promoter-reporters for an approach to the gene network underlying the circadian system. Physiological functions of Lemna clock-related homologues and the comparison with those in Arabidopsis will be discussed.

Journal

Details 詳細情報について

  • CRID
    1390282680608782848
  • NII Article ID
    130006993571
  • DOI
    10.14841/jspp.2007.0.535.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top