Functional Analysis for Circadian Clock-Related Genes of Duckweeds by Using a Transient Expression System
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- Serikawa Masayuki
- Division of Biological Science, Graduate School fo Science, Nagoya Univ.
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- Miwa Kumiko
- Division of Biological Science, Graduate School fo Science, Nagoya Univ.
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- Suzuki Sayaka
- Division of Biological Science, Graduate School fo Science, Nagoya Univ.
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- Kondo Takao
- Division of Biological Science, Graduate School fo Science, Nagoya Univ.
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- Oyama Tokitaka
- Division of Biological Science, Graduate School fo Science, Nagoya Univ.
Bibliographic Information
- Other Title
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- 一過的発現系を用いたウキクサ概日時計関連遺伝子の機能解析
Abstract
The Lemna genus in duckweeds includes two species that show photoperiodic flowering responses of a long-day type (L. gibba) and a short-day type (L. paucicostata). We tried to reveal the molecular basis for their timing systems. We isolated a number of Lemna homologues of circadian clock-related genes of Arabidopsis. We then tried establishing the methods for analysis of functions of those homologues in the Lemna plants. To monitor the circadian rhythm, we developed a bioluminescent reporter system in which the luciferase gene under a circadian promoter was introduced by using a particle bombardment method. Then, this semi-transient bioluminescence monitoring system was combined with overexpression/RNAi procedures to manipulate the expression levels of clock-related homologues. This enabled us to try various combinations of effectors/promoter-reporters for an approach to the gene network underlying the circadian system. Physiological functions of Lemna clock-related homologues and the comparison with those in Arabidopsis will be discussed.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2007 (0), 535-535, 2007
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680608782848
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- NII Article ID
- 130006993571
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed