Metabolic Profiling and Transcriptome Analysis of ABA Biosynthetic Mutant, <I>nced3</I> Under Drought Stress
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- Urano Kaoru
- Plant Mol. Biol., RIKEN PSC, RIKEN
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- Maruyama Kyonoshin
- Biol. Resource Div., JIRCAS
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- Takeda Migiwa
- Kazusa DNA Res. Inst.
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- Suzuki Hideyuki
- Kazusa DNA Res. Inst.
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- Saito Kazuki
- PSC, RIKEN Kazusa DNA Res. Inst. Grad. Sch. Pharm. Sci., Univ. Chiba
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- Shibata Daisuke
- Kazusa DNA Res. Inst.
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- Yamaguchi-Shinozaki Kazuko
- Biol. Resource Div., JIRCAS Grad. Sch. Agr. Life Sci., Univ. Tokyo/JST. CREST
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- Shinozaki Kazuo
- Plant Mol. Biol., RIKEN PSC, RIKEN
Bibliographic Information
- Other Title
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- 乾燥ストレス下でのABA生合成欠損変異体<I>nced3</I>のメタボローム・トランスクリプトーム解析
Abstract
ABA is a phytohormone that plays an essential role in drought response in plants. A lot of drought inducible genes are regulated by ABA signal and production. There are 6 genes encoding a 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme involved in the biosynthesis of ABA in Arabidopsis genome. In AtNCEDs, NCED3 was required for drought inducible ABA accumulation that is essential for drought avoidance. <br>In the present study, we performed metabolic profiling and transcriptome analysis of NCED3 knockout mutant (nced3-2) under drought stress. Metabolic profiling using GC/MS and CE/MS showed a drought-inducible accumulation of various types of amino acid, small organic compound and sugar were reduced in the nced3-2. Microarray analysis also showed that about 50% of drought-inducible genes were reduced in nced3-2. Based on these experiment, we will discuss the biological function of ABA involved in metabolic networks of plant in drought stress response.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 804-804, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680608845824
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- NII Article ID
- 130006993667
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed