Metabolic Profiling and Transcriptome Analysis of ABA Biosynthetic Mutant, <I>nced3</I> Under Drought Stress

DOI

Bibliographic Information

Other Title
  • 乾燥ストレス下でのABA生合成欠損変異体<I>nced3</I>のメタボローム・トランスクリプトーム解析

Abstract

ABA is a phytohormone that plays an essential role in drought response in plants. A lot of drought inducible genes are regulated by ABA signal and production. There are 6 genes encoding a 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme involved in the biosynthesis of ABA in Arabidopsis genome. In AtNCEDs, NCED3 was required for drought inducible ABA accumulation that is essential for drought avoidance. <br>In the present study, we performed metabolic profiling and transcriptome analysis of NCED3 knockout mutant (nced3-2) under drought stress. Metabolic profiling using GC/MS and CE/MS showed a drought-inducible accumulation of various types of amino acid, small organic compound and sugar were reduced in the nced3-2. Microarray analysis also showed that about 50% of drought-inducible genes were reduced in nced3-2. Based on these experiment, we will discuss the biological function of ABA involved in metabolic networks of plant in drought stress response.

Journal

Details 詳細情報について

  • CRID
    1390282680608845824
  • NII Article ID
    130006993667
  • DOI
    10.14841/jspp.2006.0.804.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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