CYO1/ABC2 protein, a cotyledon-specific chloroplast development factor of Arabidopsis, had thiol-disulfide reduction activity
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- Shimada Hiroshi
- Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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- Ogura Kan
- Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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- Mochizuki Mariko
- Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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- Mori Kazuaki
- Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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- Shirano Yumiko
- Boyce Thompson Institute for Plant Research
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Takamiya Ken-ichiro
- Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
Bibliographic Information
- Other Title
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- 子葉葉緑体形成因子CYO1/ABC2は茎頂分裂組織形成に必須な分子シャペロンである。
Abstract
Chloroplast development in cotyledons differs in a number of ways from that in true leaves, but the cotyledon-specific program of chloroplast biogenesis has not been clarified. The cyo1/abc2 mutant in Arabidopsis thaliana has albino cotyledons but normal green true leaves. Chloroplasts develop abnormally in cyo1/abc2 mutant plants grown in the light, but etioplasts are normal in mutants grown in the dark. CYO1/ABC2 protein localizes to the thylakoid membrane in chloroplasts, and CYO1/ABC2 protein copurified with the PSI/LHCI and PSII/LHCII complexes. CYO1/ABC2 has a C4-type zinc finger domain similar to that of Escherichia coli DnaJ. Recombinant CYO1 accelerates disulfide bond reduction in the model substrate insulin and renatures RNase A, indicating that CYO1/ABC2 has protein disulfide isomerase activity. These results suggest that CYO1/ABC2 has a chaperone-like activity required for thylakoid biogenesis in cotyledons.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2008 (0), 0172-0172, 2008
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680609017344
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- NII Article ID
- 130006993933
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed