Two-Photon Fluorescence Spectroimaging of the Thylakoid Membrane in Chlorella Chloroplasts
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- Yoshida Takahiko
- Graduate School of Science, Kyoto Univ.
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- Hasegawa Makoto
- Graduate School of Science, Kyoto Univ.
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- Ikegami Isamu
- Faculty of Pharmaceutical Sci., Teikyo Univ.
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- Terazima Masahide
- Graduate School of Science, Kyoto Univ.
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- Kumazaki Shigeichi
- Graduate School of Science, Kyoto Univ.
Bibliographic Information
- Other Title
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- 緑藻クロレラ葉緑体微細構造の二光子励起顕微蛍光スペクトル解析
Abstract
Chlorella, a green alga, has a chloroplast that contains thylakoid membrane with photosynthetic pigments both under light and dark conditions. We have studied their fine structures and spectra of subchloroplast regions by fluorescence spectromicroscope, in an effort to understand correlation between ultrastructural morphology and physiological functions of chloroplasts in general. Chlorella chloroplast seems to be an interesting control sample compared with plant chloroplast, from the viewpoint of spectromicroscopic investigation. Chlorella chloroplast is free from differentiation. It is a single chloroplast in the small cell, where light can penetrate easily. It is thus possible to quantitatively estimate the concentration of the photosynthetic pigments, by which reabsorption effects on subcellular fluorescence spectra can be well appreciated. Electron microscopic studies in the past have shown that it is free from granal stacking of thylakoid membrane. It is thus regarded as a nice control sample in comparison with plant chloroplast having granal stacking.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2008 (0), 0586-0586, 2008
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680609046656
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- NII Article ID
- 130006993979
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed