Functional Analysis Of The Plant-specific CDK-activating Kinase CDKF;1

DOI
  • Takatsuka Hirotomo
    Graduate School of Biological Sciences,Nara Institute of Science and Technology Graduate School of Science,Tokyo Univ.
  • Umeda-Hara Chikage
    Graduate School of Biological Sciences,Nara Institute of Science and Technology
  • Uchimiya Hirofumi
    Graduate School of Science,Tokyo Univ.
  • Umeda Masaaki
    Graduate School of Biological Sciences,Nara Institute of Science and Technology

Bibliographic Information

Other Title
  • 植物特異的CDK活性化キナーゼCDKF;1の機能解析

Description

CDKF;1 is a plant-specific cyclin-dependent kinase (CDK) and functions as a CDK-activating kinase (CAK) in vitro. Such a function has been previously examined with transiently expressed CDKF;1 in root protoplasts. To reveal its in vivo functions, we isolated a T-DNA insertion mutant and investigated the phenotype. The cdkf;1 mutant exhibited a dwarf phenotype and delayed growth. Root growth stopped around 7 days after germination and the expression of CYCB1-GUS almost disappeared, suggesting that CDKF;1 is required for cell division in the root meristem. We also found that the leaf blade area, cell number and cell size were smaller than those of wild-type plants. Moreover, the cdkf;1 mutant showed a lower level of DNA ploidy. On the other hand, cell division pattern during embryogenesis has not been defected in the mutant. Based on these results, we concluded that CDKF;1 plays an essential role in cell divisions during post-embryonic development.

Journal

Details 詳細情報について

  • CRID
    1390282680609138688
  • NII Article ID
    130006994121
  • DOI
    10.14841/jspp.2007.0.277.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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