Functional Analysis Of The Plant-specific CDK-activating Kinase CDKF;1
-
- Takatsuka Hirotomo
- Graduate School of Biological Sciences,Nara Institute of Science and Technology Graduate School of Science,Tokyo Univ.
-
- Umeda-Hara Chikage
- Graduate School of Biological Sciences,Nara Institute of Science and Technology
-
- Uchimiya Hirofumi
- Graduate School of Science,Tokyo Univ.
-
- Umeda Masaaki
- Graduate School of Biological Sciences,Nara Institute of Science and Technology
Bibliographic Information
- Other Title
-
- 植物特異的CDK活性化キナーゼCDKF;1の機能解析
Description
CDKF;1 is a plant-specific cyclin-dependent kinase (CDK) and functions as a CDK-activating kinase (CAK) in vitro. Such a function has been previously examined with transiently expressed CDKF;1 in root protoplasts. To reveal its in vivo functions, we isolated a T-DNA insertion mutant and investigated the phenotype. The cdkf;1 mutant exhibited a dwarf phenotype and delayed growth. Root growth stopped around 7 days after germination and the expression of CYCB1-GUS almost disappeared, suggesting that CDKF;1 is required for cell division in the root meristem. We also found that the leaf blade area, cell number and cell size were smaller than those of wild-type plants. Moreover, the cdkf;1 mutant showed a lower level of DNA ploidy. On the other hand, cell division pattern during embryogenesis has not been defected in the mutant. Based on these results, we concluded that CDKF;1 plays an essential role in cell divisions during post-embryonic development.
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2007 (0), 277-277, 2007
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390282680609138688
-
- NII Article ID
- 130006994121
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed