Direct synthesis of tZ-type cytokinin is important for gall formation

DOI

Bibliographic Information

Other Title
  • クラウンゴール形成時におけるプラスチド内でのゼアチン型サイトカイニンの直接合成の重要性

Description

Agrobacterium tumefaciens infection induces tumor formation by integrating the T-DNA region of Ti-plasmid into the plant nuclear genome. Tumors are formed because the T-DNA encodes enzymes that synthesize two plant hormones, auxin and cytokinin. We had demonstrated that a cytokinin biosynthesis enzyme, Tmr, which is encoded by the T-DNA region, is targeted to and functions in plastids of infected plant. The Tmr creates a metabolic bypass for direct synthesis of tZ-type cytokinin by using HMBDP. To reveal biological importance of the Tmr function for gall formation, we prepared Agrobacterium mutants, whose Tmr is replaced by Tzs or AtIPT1. These IPTs exhibit different subcellular localization or different substrate specificity from Tmr. Substitution of Tmr with AtIPT resulted in decrease of tZ-type cytokinin content in the tumor and tumor formation efficiency. This result suggests that direct synthesis of tZ-type cytokinin is important for gall formation. Results of Tzs-substitution will be also presented.

Journal

Details 詳細情報について

  • CRID
    1390282680609201536
  • NII Article ID
    130006994213
  • DOI
    10.14841/jspp.2009.0.0354.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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