Annotations of metabolites in the isolated vacuoles obtained from suspension cultured cells of Arabisopsis
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- Sasaki Ryosuke
- Kazusa DNA Res. Inst. CREST
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- Ohnishi Miwa
- Kobe Univ., Faculty of Science CREST
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- Anegawa Aya
- Kobe Univ., Faculty of Science CREST
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- Sugiyama Yuko
- Univ. of Hyogo CREST
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- Iijima Yoko
- Kazusa DNA Res. Inst. CREST
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- Sakurai Nozomu
- Kazusa DNA Res. Inst. CREST
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- Shibata Daisuke
- Kazusa DNA Res. Inst.
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- Mimura Tetsuro
- Kobe Univ., Faculty of Science CREST
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- Aoki Koh
- Kazusa DNA Res. Inst. CREST
Bibliographic Information
- Other Title
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- シロイヌナズナ単離液胞代謝物のLC-FTICR-MS分析データに基づく包括的アノテーション
Abstract
Vacuole of plant cells is a storage organelle of various metabolites. However, an actual diversity of metabolites in a vacuole has not been clarified. In this study, we performed a comprehensive annotation of metabolites in the isolated vacuoles from suspension cultured cell of Arabidopsis (DEEP stain) using LC-FTICR-MS, to elucidate basic metabolite profile of vacuole. A metabolite profile of the whole cell fraction was also analyzed. From the isolated vacuoles and the whole cells, 736 and 876 metabolites were detected in positive mode, respectively, and 713 and 989 metabolites were detected in negative mode, respectively. The whole cell extract contained a larger number of metabolites in a m/z range smaller than 500 Da. Interestingly, several metabolites in nucleic acid metabolism and amino acid metabolism were detected exclusively in the isolated vacuole or the whole cell. This work was partly supported by CREST, JST.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2009 (0), 0306-0306, 2009
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680609290496
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- NII Article ID
- 130006994341
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed