Cloning and Sequencing of the Genes Encoding Five Extrinsic Proteins in Diatom PSII Particles

DOI

Bibliographic Information

Other Title
  • 珪藻PSII標品に存在する5種の表在性タンパクのクローニングと解析

Description

Photosystem II (PSII) contains different extrinsic proteins among different organisms. Recently, Enami et al. succeeded in the preparation of PSII particles having high oxygen-evolving activity from a marine diatom Chaetoceros gracilis and found that the diatom PSII particles contained five extrinsic proteins, PsbO, PsbQ', PsbV, hypothetical protein and PsbU. In this study, we cloned and sequenced the genes encoding these five extrinsic proteins. Full-lengths of psbO, psbQ', hypothetical protein, and psbU cDNAs were isolated by degenerate RT-PCR and RLM-RACE. On the other hand, DNA fragment containing psbV gene was isolated using a combination of degenerate RT-PCR and inverse PCR. The results show that the former genes are encoded in the nuclear genome. After sequencing the genes, we found that all of the five extrinsic proteins have typical features for thylakoid transfer peptides. This suggests that the hypothetical protein is also one of the luminal extrinsic proteins functioning in oxygen evolution.

Journal

Details 詳細情報について

  • CRID
    1390282680609641472
  • NII Article ID
    130006994871
  • DOI
    10.14841/jspp.2007.0.331.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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