Analysis of enzymatic activities of Xyloglucan endotransglucosylase/hydrolases (XTHs) in terrestrial plants

DOI

Bibliographic Information

Other Title
  • 陸上植物におけるエンド型キシログルカン転移酵素／加水分解酵素（XTH）の機能解析

Abstract

Xyloglucans (XGs) are major components of dicot cell walls and function as cross-links between cellulose microfibrils. Xyloglucan endotransglucosylase/hydrolases (XTHs), which catalyze cleavage and reconnection of the XG cross-links, are considered to play important roles in construction and reorganization of cell walls in dicots.<br> XTHs are widely found in terrestrial plants. In Arabidopsis thaliana, Oryza sativa and the moss, Physcomitrella patens, XTHs are encoded by large gene families. This raises a question about roles of XTH in monocots and mosses. For, XG is a minor component in rice cell wall, and XGs in P. patens are, if any, distinct from that in dicots in terms of structural feature. This imply that rice and P. patens XTHs possess different roles than those of dicots.<br> To test the possibility, we analyzed their biological functions and enzymatic activities using recombinant proteins. Three rice OsXTHs exhibited transglucosylase activity and / or hydrolase activity on XG, suggesting that XG is the substrate for XTH in rice. We are now working on functions of the XTH in P. patens which lacks signal peptide and is not secreted into the cell wall.

Journal

Details 詳細情報について

  • CRID
    1390282680609741440
  • NII Article ID
    130006995011
  • DOI
    10.14841/jspp.2010.0.0365.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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