Regulation Of The Tobacco Calmodulin-binding Mitogen-activated Protein Kinase Phosphatase NtMKP1 By Wounding
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- Katou Shinpei
- International Young Researchers Empowerment Center, Shinshu Univ. Nat. Inst. of Agr. Sci.
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- Asakura Nobuhide
- International Young Researchers Empowerment Center, Shinshu Univ.
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- Onishi Yasuro
- International Young Researchers Empowerment Center, Shinshu Univ.
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- Seo Shigemi
- Nat. Inst. of Agr. Sci.
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- Mitsuhara Ichiro
- Nat. Inst. of Agr. Sci.
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- Ohashi Yuko
- Nat. Inst. of Agr. Sci.
Bibliographic Information
- Other Title
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- タバコのカルモジュリン結合性MAPキナーゼ脱リン酸化酵素・NtMKP1の傷害による制御
Abstract
The mitogen-activated protein kinases (MAPKs) are key molecules of signal transduction responses to various extracellular stimuli. In tobacco, two pathogen- and wound-induced MAPKs, WIPK and SIPK, regulate stress-induced accumulation of jasmonic and salicylic acids. MAPK phosphatases (MKPs) are negative regulators of MAPKs. We have reported that overexpression of NtMKP1, a tobacco calmodulin-binding MKP, compromised wound-induced activation of WIPK and SIPK. In this study, we investigated the changes in the levels of NtMKP1 transcripts and proteins in response to wounding. Quantitative RT-PCR analysis revealed that NtMKP1 mRNA is once decreased and then increased in response to wounding. The level of NtMKP1 protein was investigated by immunoblot analysis; however it was not detected in the wild type plants. Therefore, we used the plants expressing NtMKP1 under Cauliflower mosaic virus 35S promoter. The level of NtMKP1 protein was rapidly decreased, kept at the lower level for several hours and then returned to the basal level after wounding. These results indicated that NtMKP1 is regulated at both transcriptional and post-transcriptional levels.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2011 (0), 0156-0156, 2011
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680609917440
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- NII Article ID
- 130006995265
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed