Cyanobacterial two-component system Hik8-RpaA regulates the light/redox-responsive expression of <I>psaA</I>.

DOI
  • Midorikawa Takafumi
    Department of Biological Science, Graduate School of Sciences, Univ. of Tokyo
  • Narikawa Rei
    Graduate School of Arts and Sciences, Univ. of Tokyo
  • Ikeuchi Masahiko
    Department of Biological Science, Graduate School of Sciences, Univ. of Tokyo Graduate School of Arts and Sciences, Univ. of Tokyo

Bibliographic Information

Other Title
  • Hik8・RpaAが制御するシアノバクテリア<I>psaA</I>遺伝子の光・レドクス調節機構

Description

The psaA gene, which encodes photosystem I reaction center subunit, is the critical target of regulation in environmental responses of at least cyanobacteria. We previously reported that the OmpR-type response regulator RpaA binds to the upstream region of psaA in Synechocystis sp. PCC 6803. RpaA was phosphorylated by histidine kinase SasA in a circadian manner in Synechococcus elongatus. Hik8 (SasA) controls expression of sugar catabolic genes in Synechocystis. In this study, we examined the role of RpaA in light- or glucose- induced expression of psaA. In wild type, the psaA transcription is inactive in the dark and induced by light illumination or glucose feeding. This induction was strongly reduced by rpaA or hik8 disruption. The induction was also interrupted by an electron transport inhibitor DBMIB. Thus, it is suggested that the Hik8-RpaA system mediates signal transduction from the redox state of the electron transport chain to the transcription of psaA. The rpaA disruption also affected the light- and glucose-induced expressions of cpcB and genes for glycolysis which are under control of Hik8, even though RpaA did not directly bind to their upstream regions.

Journal

Details 詳細情報について

  • CRID
    1390282680611120768
  • NII Article ID
    130006996719
  • DOI
    10.14841/jspp.2011.0.0459.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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