Contributions of Methionine Synthase to Nyctinasty
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- Otsuka Yuki
- Fac. of Sci. & Tech. Grad. Sch., Sophia Univ.
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- Manabe Yoshiyuki
- Fac. of Sci. Grad. Sch., Tohoku Univ.
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- Kawashima Youichi
- Fac. of Chem., Sophia Univ.
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- Ueda Minoru
- Fac. of Sci. Grad. Sch., Tohoku Univ.
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- Kanzawa Nobuyuki
- Fac. of Sci. & Tech. Grad. Sch., Sophia Univ. Fac. of Chem., Sophia Univ.
Bibliographic Information
- Other Title
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- メチオニン合成酵素の就眠運動への関与
Abstract
Legume plants open their leaves early in the morning and close them in the evening. This phenomenon is called nyctinasty, which is controlled by circadian clock regulating the balance of concentrations between two endogenous bioactive substances, leaf-opening factor (LOF) and leaf-closing factor (LCF). Recent studies of Prof. M. Ueda et. al., (Tohoku University) identified a candidate for LOF binding protein in Cassia plant. Identified fragment sequences of the protein showed similarity to methionine synthase (MS). Thus, to examine whether MS is able to bind to LOF, how this binding affects MS activity, and whether MS plays the same role in other plants, we isolated cDNA clone of MS from Cassia obtusifolia. An anti-MS antibody generated against recombinant MS was used to examine the tissue distribution of MS. As a result, bacterially expressed recombinant proteins exhibited MS activity and were bound to LOF, though the activity was not changed by their binding. These data showed that MS appears to be a LOF receptor in vivo, though the role was still uncertain.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2011 (0), 0474-0474, 2011
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680611179136
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- NII Article ID
- 130006996811
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed