Molecular cloning and functional characterization of flavonoid 8-hydroxylase from <I>Lotus japonicus</I>
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- Shimada Norimoto
- Kazusa DNA Res. Inst.
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- Akashi Tomoyoshi
- Dept. Appl. Biol. Sci., Nihon Univ.
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- Aoki Toshio
- Dept. Appl. Biol. Sci., Nihon Univ.
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- Kanamori Tina
- Graduate School of Life and Env. Sci., Osaka Pref. Univ.
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- Ohta Daisaku
- Graduate School of Life and Env. Sci., Osaka Pref. Univ.
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- Aoki Koh
- Kazusa DNA Res. Inst.
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- Shibata Daisuke
- Kazusa DNA Res. Inst.
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- Suzuki Hideyuki
- Kazusa DNA Res. Inst.
Bibliographic Information
- Other Title
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- ミヤコグサ由来フラボノイド 8-水酸化酵素遺伝子の単離と機能解析
Abstract
Lotus japonicus, a model legume, accumulates 8-hydroxyflavonol derivatives which are responsible for the yellow color of flower petals. From L. japonicus petals, we had purified a flavonol glycoside, gossypin, which on hydrolysis yielded the aglycone, 8-hydroxyquercetin. A recent study showed that flavonoid 8-hydroxylase in Asteraceae is localized in the microsomal fraction and uses NADPH and FAD as cofactors, but there has been no report on the identification of flavonoid 8-hydroxylase. Thus, we attempted to isolate a cDNA of flavonoid 8-hydroxylase from L. japonicus. <br>To isolate cDNA, we searched EST data and have selected an FAD-containing monooxygenase-like protein which was only found in EST data derived from flower buds. The heterologously expressed protein catalyzes the C-8 hydroxylation of flavonol, quercetin. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. We will discuss its catalytic properties in this annual meeting.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2011 (0), 0387-0387, 2011
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680611288320
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- NII Article ID
- 130006996979
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed