DDBJ Read Annotation Pipeline: A cloud computing based pipeline for high-throughput analysis of new generation sequencing data
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- Nagasaki Hideki
- CIB-DDBJ, Nat. Inst. of Genetics
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- Mochizuki Takako
- CIB-DDBJ, Nat. Inst. of Genetics
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- Kaminuma Eli
- CIB-DDBJ, Nat. Inst. of Genetics
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- Watanabe Shigeki
- CIB-DDBJ, Nat. Inst. of Genetics
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- Kodama Yuichi
- CIB-DDBJ, Nat. Inst. of Genetics
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- Saruhashi Satoshi
- CIB-DDBJ, Nat. Inst. of Genetics
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- Sugawara Hideaki
- CIB-DDBJ, Nat. Inst. of Genetics
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- Takagi Toshihisa
- CIB-DDBJ, Nat. Inst. of Genetics Dept. Computational Biology, Grad. Sch. Frontier Sciences, University of Tokyo
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- Okubo Kousaku
- Dept. Computational Biology, Grad. Sch. Frontier Sciences, University of Tokyo DBCLS
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- Nakamura Yasukazu
- CIB-DDBJ, Nat. Inst. of Genetics
Bibliographic Information
- Other Title
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- DDBJ Read Annotation Pipeline : 新型シーケンサ由来配列のクラウド型パイプライン
Description
The DNA Data Bank of Japan (DDBJ) released DDBJ Sequence Read Archive (DRA), an archive database of raw data for New Generation Sequencing (NGS) reads. <br>The huge and short read data generated by the sequencers suffer biologists to require computational hardware and bioinformatics skills. DDBJ has been started the service of a cloud computing based analytical pipeline for high-throughput annotation for NGS reads named as DDBJ Read Annotation Pipeline (http://p.ddbj.nig.ac.jp/). <br>The features of the pipeline are as follows.<br>1) Application tools for various NGS platforms (illumina, Roche/454 and Life technology) are available.<br>2) Fundamental statistics such as quality scores or sequence depth is calculated via output files with uniform format.<br>3) DDBJ Supercomputer is utilized with remote control via the pipeline web server under scalable cloud computing manner.<br>In the annotation stage of the pipeline, various functions of piled sequencing reads (detecting SNPs and indels, counting gene expression levels, and so on), genome-wide visualization of alignment results are introduced.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2011 (0), 0828-0828, 2011
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680612736896
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- NII Article ID
- 130006998625
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed