Accumulation of Tree Pollen Chimera 7 (TPC7) induces novel storage organelle formation in rice endosperm
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- Wang Shuyi
- National Institute of Agrobiological Sciences (NIAS)
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- Takahashi Hideyuki
- National Institute of Agrobiological Sciences (NIAS)
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- Kawakatsu Taiji
- National Institute of Agrobiological Sciences (NIAS)
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- Takaiwa Fumio
- National Institute of Agrobiological Sciences (NIAS)
Bibliographic Information
- Other Title
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- イネ胚乳においてTree Pollen Chimera 7 (TPC7)タンパク質は新規貯蔵オルガネラ形成を誘導する
Abstract
Bet v 1 family proteins of the Fagales order are the major pollen allergens. Fourteen genes of the Bet v 1 family were randomly recombined in vitro by means of DNA shuffling. A resultant low allergenic Tree Pollen Chimera 7 (TPC7) has high immunogenicity against multiple Fagales pollen allergy in vitro and in vivo. Towards development of rice seed-based edible vaccine against multiple Fagales pollen allergy, we generated transgenic rice that accumulate TPC7 in the endosperm. TPC7 high accumulating lines were screened by immunoblot using anti-TPC7 antibody. TPC7 in high accumulating lines was detectable as two visible bands on CBB-stained SDS-PAGE gel. After glycosidase reaction, two bands became a single band, indicating that rice seed-derived TPC7 was glycosylated. In rice endosperm cells, seed storage protein prolamins are deposited in the ER-derived protein body-I (PB-I), and glutelins and globulin are deposited in the storage vacuole PB-II. Since TPC7 was deposited into the novel organelle other than PB-I or PB-II, we termed this as TPC7 body. We will report biochemical properties of TPC7 protein in the transgenic rice and the character of TPC7 body.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2011 (0), 0905-0905, 2011
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680612990592
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- NII Article ID
- 130006998945
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed