Real-Time Visualization of Double-Strands Break in DNA : Quantitative Analysis on the Protective Effect of Vitamin C
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- Yoshikawa Yuko
- Faculty of Education for Future Generations, International Pacific University
Bibliographic Information
- Other Title
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- DNA二重鎖切断反応の可視化 : ビタミンCによる抑制効果の定量的計測
- DNA 2ジュウサ セツダン ハンノウ ノ カシカ ビタミン C ニ ヨル ヨクセイ コウカ ノ テイリョウテキ ケイソク
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Abstract
Direct attack to genomic DNA by reactive oxygen species causes various types of lesions. The most significant lesion is considered to be an unrepaired double-strand break that can lead to fatal cell damage. Using fluorescence microscopy, we directly observed double-strand breaks of DNA in reconstituted chromatin stained by a fluorescent cyanine dye, YOYO, in solution. YOYO is known to have the ability to photo-cleave DNAs by generating reactive oxygen species. We also investigated the effect of an antioxidant vitamin, vitamin C, on preventing double-strand breaks in a quantitative manner. We showed that doublestrand breaks can be monitored at the level of individual DNA molecules, and that the kinetics of the breakage reaction can be deduced from such single-molecule observation. We found that DNA is protected against double-strand breaks by the addition of vitamin C.
Journal
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- VITAMINS
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VITAMINS 81 (7), 297-302, 2007
THE VITAMIN SOCIETY OF JAPAN
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Details 詳細情報について
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- CRID
- 1390282680676805120
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- NII Article ID
- 110006368520
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- NII Book ID
- AN00207833
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- ISSN
- 2424080X
- 0006386X
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- NDL BIB ID
- 8902181
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed