Vitamin B_6 Analysis : Current status and Challenges

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  • Yagi Toshiharu
    Agricultural Science Program, Graduate School of Integral Art and Sciences, Kochi University

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Other Title
  • ビタミンB_6測定法の現状と課題(<特集>ビタミン測定法の現状と課題[I])
  • ビタミンB6測定法の現状と課題
  • ビタミン B6 ソクテイホウ ノ ゲンジョウ ト カダイ

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Abstract

Natural vitamin B_6 consists of six compounds which are pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5'-phosphate, pyridoxal 5'-phosphate, and pyridoxamine 5'-phosphate. They show the same nutritional efficiency. Plant-derived foods contain pyridoxine-β-glucoside, a storage form of vitamin B_6. These compounds have chemically distinct functional groups. Many methods have been developed to determine them. Current application status and characteristics of microbiological methods, spectroscopic, fluorometric, electrochemical, and capillary electrophoretic methods, and liquid chromatographic, including HPLC, methods are commented. Advantages and disadvantages of several sample preparation methods for analysis of vitamin B_6 compounds are also described. Finally, a principle and protocols of a challenging method, all enzymatic HPLC method, which is applicable for determination of the individual vitamin B_6 compounds and pyridoxine-β-glucoside, is described.

Journal

  • VITAMINS

    VITAMINS 85 (3), 116-126, 2011

    THE VITAMIN SOCIETY OF JAPAN

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