Effect of Exercise on the Elevation of HSP70 in Rats Fed a Diet Containing Different Amounts of Vitamin B_6

OKADA Mitsuko, Uji Masami, KIRINO Tomomi, KUGIMOTO Kaori, KIYONAGA Naoko, OTSUBO Husako, Takeshita-IMOU Keiko, Kaku-OHKURA Shihoko

doi:10.20632/vso.78.9_429

The effect of exercise on the elevation of HSP70 was studied in rats fed a different diet on the B_6 content (as pyridoxine 1.5 mg/kg diet or 7.5 mg/kg diet). Minimum requirement of B_6 in the diet is known to be about 1.5 mg/kg diet in 20 % casein diet. Rats were divided into 4 groups in each series and assigned as Sc (sedentary control), Se (sedentary-exhaustion), Ec (training control), and Ee (trainingexhaustion). HSP70 was determined in the heart, liver and gastrocnemius muscle (red and white portions). HSP70 in the heart was not changed in all groups of both series. In the liver HSP70 was elevated to very high levels in Sc and Ee groups in the rats fed the high level of B_6, and where as a little in Ee group in rats fed low level of B_6. The levels of inductions were in the muscle not so high very similar but as observed in the liver. AST (aspartate aminotransferase) activity was significantly elevated in Ec and Ee for the liver, and in the three groups for the kidney and muscle (red portion) in the rats fed the high level of B_6. On the contrary in all tissues in all groups fed low level of B_6, AST activity was not changed at all. Thus the low induction of HSP70 in the tissues, especially in the liver of rats fed the low B_6 diet, may suggest impairment of amino acid metabolism and, consequently, insufficiency of energy supply.

Effect of Exercise on the Elevation of HSP70 in Rats Fed a Diet Containing Different Amounts of Vitamin B_6

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