Selective collection of catecholaminergic (CA) neurons in the brain and its application to functional analyses using tyrosine hydoroxylase (TH)–green fluorescent protein (GFP) transgenic mice

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  • 脳内カテコールアミンニューロンの選択的収集と機能解析への応用–チロシン水酸化酵素‐緑色蛍光蛋白トランスジェニックマウスを用いた検討

Abstract

CA neurons are involved in a wide spectrum of physiological functions in the brain including sensory, motor, emotional, autonomic and endocrine regulation. Most CA neurons are localized in the brainstem and hypothalamic regions and typically make clusters of cells, among which the noradrenergic (A1, A2, A6) and dopaminergic (A9, A10, A12) neurons predominate. In order to explore functional roles of these neurons, we sought to collect CA neurons selectively using TH-GFP transgenic mice in which GFP expression was driven under TH-promoter. Fetal (E14.5, E16.5, or E18.5) brain was extracted, and neurons were dispersed after treating with trypsin, then GFP-positive cells were sorted out by flow-cytometry (FACS). RNA was extracted from the GFP-positive (TH) neurons, reverse-transcribed, and analyzed by PCR. [J Physiol Sci. 2006;56 Suppl:S112]

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Details 詳細情報について

  • CRID
    1390282680705248000
  • NII Article ID
    130005448324
  • DOI
    10.14849/psjproc.2006.0_112_2
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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