Establishment of a urine bilirubin confirmation method

DOI
  • OHNO Akemi
    Central Clinical Laboratory, Keio University Hospital
  • NAGANO Katsutoshi
    Department of Clinical Laboratory, University Hospital of Medicine, Tokyo Medical and Dental University
  • YAMAGATA Junko
    Central Clinical Laboratory, Keio University Hospital
  • NOGUCHI Masayo
    Central Clinical Laboratory, Keio University Hospital
  • SHIBATA Ayako
    Central Clinical Laboratory, Keio University Hospital
  • KIKUCHI Haruhito
    Department of Laboratory Medicine, School of Medicine, Keio University
  • MURATA Mitsuru
    Department of Laboratory Medicine, School of Medicine, Keio University

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Other Title
  • 尿試験紙ビリルビン検査の偽陽性確認試験の検討

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Description

Because the strips used for the urine bilirubin test by the diazo coupling method often show a false positive result, confirmatory examinations of bilirubin true positivity are necessary. To determine urine bilirubin, there are the Rosin method, the Harrison method, and the Watson-Howkinson method, which are based on oxidation reaction. We evaluated these methods, including the procedures of the Harrison and Watson-Howkinson methods, to determine the most suitable confirmation method. As a result, we chose the Watson-Howkinson method (W method-C). In the W method-C, the preservation of the absorption pad was easy, and the procedure was simple, easy, and required a short time and a small sample volume. Then, we examined the W method-C in terms of the absorption pad to be used, the smallest sample volume, the detection limit, and the coincidence rate with Ictotest. We concluded that One-Shot Dry was the most suitable absorption pad, because the color that developed was easy to judge with only three drops of urine. The rate of agreement with Ictotest was good at 92.6%. The W method-C is also cheap, and thus it can sufficiently be used as a confirmation test of urine bilirubin on a routine basis.

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