Usefulness of immunochromatographic assay kit for the detection of <i>Legionella</i> antigen in urine specimens: Comparison of performance between a newly developed kit and three existing kits

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  • YAMAGUCHI Ikuo
    Department of Clinical Laboratory, Toyohashi Municipal Hospital
  • KINOSHITA Ikuya
    Department of Clinical Laboratory, Toyohashi Municipal Hospital
  • SAITO Tomoe
    Department of Clinical Laboratory, Toyohashi Municipal Hospital
  • YAMAMOTO Masaru
    Department of Clinical Laboratory, Toyohashi Municipal Hospital
  • YAMAMOTO Keiko
    Department of Clinical Laboratory, Toyohashi Municipal Hospital
  • SHIGEHARA Yaeko
    Department of Clinical Laboratory, Toyohashi Municipal Hospital
  • ITO Yumi
    Department of Clinical Laboratory, Toyohashi Municipal Hospital

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  • イムノクロマト法による尿中レジオネラ抗原検出試薬における有用性の検討―新たに開発された試薬と既存の3試薬の性能比較―

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Abstract

An immunochromatographic assay kit is frequently used in the diagnosis of legionellosis. We evaluated the usefulness of Immunocatch®-Legionella, a newly developed immunochromatographic assay kit for the detection of the Legionella antigen. The kit showed a higher detection sensitivity than BinaxNOW® Legionella and Check-Legionella, and showed an equivalent detection sensitivity to Q-line Kyokuto Legionella. In addition, Immunocatch®-Legionella as well as Q-line Kyokuto Legionella showed the most rapid determination. A total of 100 specimens from patients with pneumonia symptoms were tested using these immunochromatographic assay kits. The positive agreement, negative agreement, and overall agreement rates of Immunocatch®-Legionella and BinaxNOW® Legionella were 100% (1/1), 99.0% (98/99), and 99.0% (99/100), respectively. One case was negative as determined using BinaxNOW® Legionella, but was positive as determined using Immunocatch®-Legionella. The use of a concentrated specimen of this case resulted in a positive result using BinaxNOW® Legionella. These results suggest that BinaxNOW® Legionella has an insufficient sensitivity, and the determination using it may lead to a false-negative result. Immunocatch®-Legionella has a simpler procedure and higher sensitivity than the other kits. It also showed good performance with clinical specimens. In conclusion, Immunocatch®-Legionella is a clinically useful rapid diagnostic kit for the detection of Legionella antigen.

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