Evaluation of anti-<i>Treponema pallidum</i> antibody reagents based on recombinant antigens by latex agglutination test

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  • ラテックス凝集法によるリコンビナント抗原を用いた梅毒TP抗体試薬の検討

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Abstract

Mediace TPLA (Kyokuto Pharmaceutical Industrial Co., Ltd) has been used at our hospital as a diagnostic reagent for syphilis by the latex agglutination method. However, we often encountered high false-positivity rates owing to the influences of chyle and hemolysis. In this study, we evaluated anti-Treponema pallidum antibody reagents based on recombinant antigens from three manufacturers (Fujirebio Inc., Denka Seiken Co., Ltd., and Shino-Test Corporation). Good results were obtained in terms of reproducibility, prozone test, and other types of test. High sensitivity and specificity were obtained; in particular, the specificity was comparable to that obtained by the FTA-ABS method. It is expected that the required frequency of confirmatory testing by the TP antigen neutralization test and other methods will be reduced with the use of the anti-T. pallidum antibody reagents based on recombinant antigens in place of those based on antigens prepared from purified bacterial cells.

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