RBCs remaining in supernatant and RBC morphology after urinary sediment preparation

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  • TERAOKA Chiori
    Department of Clinical Laboratory, Tottori University Hospital
  • MIYANAGA Emiko
    Department of Clinical Laboratory, Tottori University Hospital
  • YAMASHITA Noriko
    Department of Clinical Laboratory, Tottori University Hospital
  • IKENAKA Ryoko
    Department of Clinical Laboratory, Tottori University Hospital
  • HARA Ayako
    Department of Clinical Laboratory, Tottori University Hospital
  • MOTOKURA Toru
    Department of Clinical Laboratory, Tottori University Hospital Division of Clinical Laboratory Medicine, Department of Pathophysiological and Therapeutic Science, Tottori University Faculty of Medicine

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Other Title
  • 尿沈渣標本作成後の上清中の赤血球残存率と赤血球形態

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Several factors may cause errors in the urinary sediment test, including centrifuge operation and supernatant removal. Previously, we reported a discrepancy between red blood cell (RBC) counts from flow cytometric analyses of noncentrifuged urine samples and urinary sediments after centrifugal separation, which was identified by basic evaluation at the entrance of the automated urine particle analyzer. To investigate the cause of this discrepancy, we examined RBCs remaining in the su2pernatant and RBC morphology after urinary sediment preparation. A significant amount of RBCs was observed to remain in the supernatant after centrifugal separation, leading to the above discrepancy. In addition, we found that RBCs that were judged “mixed” or “dysmorphic” by flow cytometric analysis were more likely to remain in the supernatant compared with RBCs that were judged “isomorphic”.

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