Enantioselective two-dimensional high-performance liquid chromatographic determination of amino acids; Analysis and physiological significance of ᴅ-amino acids in mammals
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- MIYOSHI Yurika
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- OYAMA Tsubasa
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- ITOH Yusuke
- Graduate School of Pharmaceutical Sciences, Kyushu University
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- HAMASE Kenji
- Graduate School of Pharmaceutical Sciences, Kyushu University
書誌事項
- タイトル別名
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- Enantioselective Two-Dimensional High-Performance Liquid Chromatographic Determination of Amino Acids; Analysis and Physiological Significance of D-Amino Acids in Mammals
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説明
D-Amino acids are now increasingly recognized as physiologically functional molecules and biomarkers in mammals. However, the amounts of D-amino acids are extremely low in most cases, thus highly sensitive and selective analytical methods are practically essential. In the present review article, enantioselective two-dimensional high-performance liquid chromatographic (2D-HPLC) methods are introduced and their applications to the determination of D-amino acids in mammalian samples are described. For the sensitive determination, amino acid enantiomers are derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and are determined by a fluorescence detector. The 2D-HPLC system consists of the reversed-phase non-enantioselective, but chemoselective separation of NBD-amino acids in the first dimension, and the sequential chiral separation in the second dimension. By using the system, neuroactive D-amino acids (D-Ser and D-Ala) were determined in the brain of various mammalian species and the regulation of their intrinsic amounts are also demonstrated. The physiological and diagnostic meanings of the trace amounts of D-Ser are also discussed. The 2D-HPLC systems for the acidic D-amino acids (D-Asp and D-Glu), proline analogues (D-Pro, trans- and cis-4-hydroxy-D-Pro) and hydrophilic D-amino acids (D-Arg, D-Asn, D-Asp, D-Gln, D-Glu, D-His, D-Ser, D-allo-Thr and D-Thr) are also shown.
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 35 (1), 49-57, 2014
クロマトグラフィー科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680721415424
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- NII論文ID
- 130004684359
- 40020019804
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- NII書誌ID
- AA1137755X
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- COI
- 1:CAS:528:DC%2BC2cXovFagsrc%3D
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 025349922
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可