Influences of Analyte Injection Volumes and Concentrations on Capillary Chromatography Based on Tube Radial Distribution of Carrier Solvents under Laminar Flow Conditions

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  • Tanigawa Yusuke
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
  • Jinno Naoya
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
  • Hashimoto Masahiko
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
  • Tsukagoshi Kazuhiko
    Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

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A capillary chromatography called the tube radial distribution chromatography (TRDC) system has been developed using an open capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture carrier solution. In this study, we examined the effects of the analyte injection volume (injection time) and concentration on the chromatograms in the TRDC system using a fused-silica capillary tube (50 µm i.d. and 100 cm effective length) and a water-acetonitrile-ethyl acetate mixture carrier solution (volume ratio, 3: 8: 4). Analyte solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid (1 mM each) were injected into the system with various injection times of 10-1500 s from a height of 20 cm by gravity. They were separated and detected in this order with good reproducibility up to an injection time of 150 s. The analyte solutions (0.075-3.0 mM) were analyzed with the definite injection time of 30 s from a height of 20 cm by gravity. They were separated and detected with a baseline separation and their calibration curves showed linearity up to 1.5 mM. It was confirmed that the TRDC system worked well as a quantitative analysis.

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  • CHROMATOGRAPHY

    CHROMATOGRAPHY 32 (3), 135-140, 2011

    クロマトグラフィー科学会

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