Screening of DNA Markers that Distinguish between Perennial Ryegrass (Lolium perenne) and Italian Ryegrass (L. multiflorum)
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- Tobina Hiroyuki
- United Graduate School of Agricultural Science, Gifu University (Shizuoka University)
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- Yamashita Masayuki
- Faculty of Agriculture, Shizuoka university
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- Koizumi Atsuhiro
- Faculty of Agriculture, Shizuoka university
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- Fujimori Masahiro
- Yamanashi Prefectural Dairy Experiment Station
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- Takamizo Tadashi
- National Institute of Livestock and Grassland Science
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- Hirata Mariko
- Japan Grassland Agriculture & Forage Seed Association
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- Sasaki Tohru
- Japan Grassland Agriculture & Forage Seed Association
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- Yamada Toshihiko
- Field Science Center for Northern Biosphere, Hokkaido university
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- Sawada Hitoshi
- Faculty of Agriculture, Shizuoka University
Bibliographic Information
- Other Title
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- ペレニアルライグラス(Lolium perenne)とイタリアンライグラス(L. multiflorum)を識別するDNAマーカーの選抜
- ペレニアル ライグラス Lolium perenne ト イタリアン ライグラス L multiflorum オ シキベツスル DNA マーカー ノ センバツ
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Abstract
Sequence information from genomic clones containing SSR-motifs was used to design primer pairs for the amplification of SSR-based DNA markers. The markers were screened to distinguish between perennial ryegrass and Italian ryegrass. From a total of 24 primer pairs, nine fragments, amplified by six primer pairs, were identified as species-distinguishable markers. Six of these fragments were assessed for their frequencies in each of : perennial ryegrass, Italian ryegrass and hybrid rygrass. Significant differences in the frequencies of each fragment were observed between perennial ryegrass and Italian ryegrass. In hybrid ryegrass, the frequency of individuals having both perennial ryegrass and Italian ryegrass-specific DNA fragments was high. A high frequency of hybrid ryegrass individuals had both perenial ryegrass-specific nuclear DNA fragments and Itarian ryegrass-specific chloroplast DNA fragments. Cluster analysis revealed that hybrid ryegrass varieties could be separated into two clusters. We conclude that these markers would be suitable to distinguish between perennial ryegrass and Italian ryegrass, and to detect hybridization in a population of ryegrass.
Journal
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- Japanese Journal of Grassland Science
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Japanese Journal of Grassland Science 53 (2), 138-146, 2007
Japanese Society of Grassland Science
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Details 詳細情報について
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- CRID
- 1390282680729859072
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- NII Article ID
- 110006368503
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- NII Book ID
- AN00194108
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- ISSN
- 21886555
- 04475933
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- NDL BIB ID
- 8914769
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed