Effects of Decapitation, Triiodobenzoic Acid and Cycloheximide on the Mitotic Activities in Seminal Roots of Zea mays L.

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  • 根冠切除,トリヨード安息香酸およびシクロヘキシミドがトウモロコシの種子根の根端細胞の分裂頻度に及ぼす影響
  • コンカン セツジョ トリヨード アンソクコウサン オヨビ シクロヘキシミド ガ

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Abstract

Seedlings of Zea mays L. cv. Great Bell were grown in aerated liquid cuture under four treatments ; control, removing root cap from seminal root, additions of 10^<-7>M triiodo-benzoic acid (TIBA) and 5×10^<-6>M cycloheximide (CHM) into culture solution respectively. The seminal roots sampled were dehydrated, embedded in paraffin cake, cut into continuous sections in both longitudinal and transversal directions and stained for mitotic investigation. Mitosis in the mitotic zone of the roots was examined microscopically. Results obtained are as follows ; 1. Mitosis was active between 200 to 800μm from the root apices in both the control and the TIBA treated plants and it was ceased beyond 1200μm. 2. In the decapped roots, whose root caps were removed, mitosis was active between 200 to 1000μm from the root apices and no further cell division occurred beyond 1400μm. This means that the mitotic activities were kept 100 to 200μm further back as compared to those of the control and the TIBA treated plants. 3. Mitotic activities were active in both the stele and the cortex of the decapped and the TIBA treated roots. 4. An asymmetrical distribution of mitotic activities in the mitotic zone in the roots was found especially in the decapped and the TIBA treated roots. From the results mentioned above, it is suggested that the mechanism of the rotation growth of the root is originated in the asymmetrical distribution of mitotic activities in the root and that the root curvature comes from the excessive accumulation of auxin within the root tissues resulting in ethylene formation. It was possible to straighten the root curvature by either removing root cap, TIBA application or applying CHM.

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