Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components

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  • KOIKE Masanori
    Laboratory of Forage Crop Science, Obihiro University of Agriculture and Veterinary Medicine
  • NANBU Kouhei
    Laboratory of Forage Crop Science, Obihiro University of Agriculture and Veterinary Medicine
  • SHIMADA Tohru
    Laboratory of Forage Crop Science, Obihiro University of Agriculture and Veterinary Medicine

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Other Title
  • アルファルファとVerticillium albo-atrumの相互作用 : 菌培養濾液と菌体細胞壁成分に対するin vitroの反応
  • アルファルファとVerticillium albo-atrumの相互作用--菌培養濾液と菌体細胞壁成分に対するin vitroの反応〔英文〕
  • アルファルファ ト Verticillium albo-atrum ノ ソウゴ

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Interactions between alfalfa and Verticillium albo-atrum were examined in terms of the following aspects:growth of V. albo-atrum on callus;growth of callus on the medium containing culture filtrate of V. albo-atrum and production of antifungal substances in callus treated with cell wall components (elicitor) from V. albo-atrum. Mycelial growth of V. albo-atrum was likely to be suppressed on the callus from resistant cultivars (Vertus and 5444), accompanied by strong browning. The growth of callus from Vertus (resistant, R) and Kitawakaba (susceptible, S) were vigorous on the medium containing 0.5 or 1% culture filtrate, while those from 5444 (R) and Europe (S) were inhibited. Antifungal substances were detected in all cultivars treated with cell wall components, with concentrations being higher in resistant than in susceptible cultivars. These results suggest that antifungal activity in the callus extracts indicated by the inhibition of germ tube elongation of fungal conidia is the best criterion for the selection of resistant cultivars.

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