Medium Conditions for In Vitro Selection of Aluminum-tolerant Cells of Alfalfa (Medicago sativa L.)

DOI JASI Web Site

Bibliographic Information

Other Title
  • アルファルファ(Medicago sativa L.)のアルミニウム耐性選抜における培地条件
  • アルファルファ(Medicago sativa L.)のアルミニウム耐性選抜における培地条件〔英文〕
  • アルファルファ Medicago sativa L.ノ アルミニウム タイセイ

Search this article

Description

In order to establish suitable medium conditions for in vitro selection of aluminum (Al)-tolerant cells of alfalfa, the effects of the concentration of phosphate, pH and the concentration of Al in the medium were examined. With low concentrations of phosphate in the MS medium, the growth of cells in suspention culture was greatly suppressed. Addition of phosphate at intervals of two days accelerated proliferation of suspension-cultured cells when 1/8, 1/4 and 1/2 of the standard concentrations of KH_2PO_4 were added in this way. Proliferation of cells in suspension culture in standard MS medium was more rapid than that in MS with 1/10 KH_2PO_4 at all pH values tested. In standard MS, the proliferation of suspension-cultured cells increased with increases in pH from 3.0 to 4.5. In contrast, MS with 1/10 KH_2PO_4 and pH 4.0 gave the highest proliferation rate of suspension-cultured cells. Wet weight of the cells in suspension on the seventh day after culture initiation increased in proportion to the increase in the wet weight of cells added at culture initiation. However, the increase in wet weight per gram of cells added at culture initiation was highest with 0.5 g of cells per 50 ml of liquid medium. The increase in wet weight of suspension-cultured cells up to the fourth day after culture initiation was suppressed with increased concentrations of Al in MS medium with 1/10 KH_2PO_4. There were no differences in packed volume of suspension-cultured cells between the control and cells cultured in 0.05 or 0.1 mM Al_2 (SO_4)_3・18 H_2O on the eighth day after culture initiation. However, proliferation of suspension-cultured cells was suppressed at 0.2, 0.4 and there was no proliferation at 0.8 mM Al_2 (SO_4)_3・18 H_2O. Residual Al in the medium in treatments with initial concentrations of 0.05, 0.1, 0.2 and 0.4 mM Al_2 (SO_4)_3・18 H_2O was almost absent one day after culture initiation. We conclude from the proliferation of suspension-cultured cells, the Al content of cells and residual Al in the medium, that MS medium with Al concentrations of 0.2 mM to 0.4 mM Al_2 (SO_4)_3・18 H_2O will be the most suitable for future attempts to isolate Al-tolerant cells.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top