The Effect of Abscisic Acid on the Freezing Tolerance in Lolium multiflorum Lam. and L. temulentum L.

  • TASE Kazuhiro
    Hokuriku National Agricultural Experiment Station
  • FUJII Hiroki
    (Present address)Hokkaido Prefectural Konsen Agricultural Experiment Station

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  • Lolium multiflorum Lam.とL. temulentum L.の耐凍性に関するABAの影響
  • Effect of Abscisic Acid on the Freezing

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Abstract

To clarify the effect of abscisic acid (ABA) on the development of freezing tolerance in Lolium multiflorum Lam. and L. temulentum L., we investigated the optimum exogenous ABA concentration for acquiring the freezing tolerance in seedlings, and compared the changes of freezing tolerance and endogenous ABA concentration by the ABA treatment with those by the hardening treatment. The highest level of freezing tolerance, based on the estimation of LT_<50> value (the temperature required to kill 50% of the seedlings), was achieved by treatment with 7.5×10^<-5>M ABA as foliar spray at intervals of 12h at normal growth temperature in either species. A comparison of ABA (7.5×10^<-5>M)-induced and hardening-induced freezing tolerance indicated that the ABA treatment increased freezing tolerance in 14 days, but did not completely substitute for the hardening. The ABA treatment also resulted in more rapid and great accumulation of endogenous ABA, as compared with the hardening treatment. Since the relationship between such an accumulation of endogenous ABA and the increase of freezing tolerance was not parallel, it is suggested that the development of freezing tolerance may not be dependent on endogenous ABA levels alone.

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