Application of Multi-PK antibodies to protein kinase studies

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  • マルチPK抗体を利用したプロテインキナーゼ研究

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<p>Various cellular events are regulated through protein phosphorylation mediated by protein kinases. These protein kinases are known to be involved in the pathogenesis of many diseases. Although 518 protein kinase genes were identified in the human genome, it remains unclear how many and what kind of protein kinases are expressed and activated in cells under varying situations. To investigate cellular phosphorylation signaling, we established three hybridoma cell lines (M1C, M8C, YK34) producing monoclonal antibodies, designated Multi-PK antibodies that can recognize multiple protein kinases in diverse biological species. Specifically, the M1C and M8C antibodies recognize Ser/Thr kinases and the YK34 antibody detects Tyr kinases. Using Multi-PK antibodies, newly analytical methods were developed: a profiling method for analysis of protein kinase expression in cells; a method for protein kinase identification by 2D-PAGE in combination with cyanogen bromide digestion of protein kinases; and an analytical method for intracellular protein kinase expression and phosphorylation state. In addition, we introduce three applications of Multi-PK antibodies to identify and characterize the protein kinases involved in epigenetics, glucotoxicity in type 2 diabetes, and pathogenesis of ulcerative colitis. In this review, we focus on the recently developed technologies for kinomics studies using the powerful analytical tools of Multi-PK antibodies.</p>

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