血清Glutamic-Oxalacetic TransaminaseおよびMalic Dehydrogenase活性のジアゾニウム塩を用いる新簡易定量法

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タイトル別名
  • SIMPLIFIED ASSAY METHODS OF GLUTAMIC OXALACETIC TRANSAMINASE AND MALIC DEHYDROGENASE ACTIVITIES IN SERUM, USING ZINC SALT OF 6-BENZAMIDO-4-METHOXY-m-TOLUIDINE DIAZONIUM CHLORIDE
  • ケッセイ Glutamic-Oxalacetic Transaminase オヨビ Malic Dehydrogenase カッセイ ノ ジアゾニウムエン オ モチイル シン カンイ テイリョウホウ

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Bivalent metal salts, such as Zn, Mg, Mn, Cd etc. of 6-benzamido-4-methoxy-m-toluidine diazonium chloride are more stable than its free diazonium chloride. These salts react easily with oxalacetate at room temperature in alkaline medium to form the colour substance having the maximum optical density at 520 mμ. Using these zinc salts as the colour developing reagents for oxalacetate, we established the routine assay method of glutamic-oxalacetic transaminase, and malic dehydrogenase activities in serum. Upon measuring GOT activity in serum of patients by three different methods (diazonium salt method, Karmen method and Reitman・Frankel method), we came to a conclusion that this new diazonium method was very advantageous to Reitman・Frankel method in accuracy and simplicity for clinicians.

収録刊行物

  • ビタミン

    ビタミン 31 (1), 72-76, 1965

    公益社団法人 日本ビタミン学会

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