Sporolactobacillus inulinusの最少培地の調製

DOI
  • 鈴木 洋一
    東京農業大学農芸化学科応用微生物学研究室
  • 豊田 泰
    東京農業大学農芸化学科応用微生物学研究室
  • 小崎 道雄
    東京農業大学農芸化学科応用微生物学研究室
  • 北原 覚雄
    東京農業大学農芸化学科応用微生物学研究室

書誌事項

タイトル別名
  • Studies on the Preparation of Minimum Medium for Growth of Sporolactobacillus inulinus

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説明

During the course of study to prepare a minimum growth medium for Sporolactobacillus inulinus, necessity of minute amount of thiamine and stimulatory effect of p-aminobenzoic acid (PABA) were demonstrated. According to the result of this study a basal minimum medium (BMM) was proposed essential requirement of minute amount thiamines stimulated by PABA in the preceding paper of this series. Curiously, however, it was found that when a natural medium (GYP) was used for preculture instead of the synthetic medium, the tested microorganism could not grow in the BMM. This shows that the BMM is not always satisfactory. Hence, the growth-promoting effects of various amino acids were reexamined. The results showed that cysteine was indispensable and, on the contrary, PABA became unnecessary for growth of this organism. Thus the minimum medium for Sporolactobacillus inulinus was finally determined as follows : glucose, sodium acetate, minerals, biotin, calcium pantothenate, thiamine, valine, leucine, glutamic acid and cysteine.

収録刊行物

  • ビタミン

    ビタミン 51 (3), 107-110, 1977

    公益社団法人 日本ビタミン学会

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