ラット肝アポTyrosine transaminaseを用いるピリドキサールりん酸の定量法の検討

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タイトル別名
  • Studies on Quantitative Determination of Pyridoxal Phosphate by Apo-tyrosine Transaminase of Rat Liver
  • ラット ギモ アポ Tyrosine transaminase オ モチイル ピリドキサールリンサン ノ テイリョウホウ ノ ケントウ

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抄録

A new enzymatic method for determination of pyridoxal phosphate was studied by using the induced tyrosine transaminase of liver of rats administered with hydrocortisone. The enzyme preparation was obtained from rat liver homogenate with dialysis and heat treatment which rendered it to respond only to pyridoxal phosphate, but neither to pyridoxine phosphate nor to pyridoxamine phosphate. Possible range of determination in the samples was from 10 to 100mμg using the standard curve of authentic pyridoxal phosphate. The enzyme solution can be used for the assay for 2 months, if kept below-20℃. At least 60 estimations of the samples can be performed by the enzyme solution obtained from one rat liver. (Received Jan. 13,1971)

収録刊行物

  • ビタミン

    ビタミン 43 (3.4), 139-143, 1971

    公益社団法人 日本ビタミン学会

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