Enzymatic Formation of New L-Ascorbic Acid Glycosides (Preliminary Report)

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  • MIYAKE Toshio
    Institute for Agricultural and Biological Sciences, Okayama University
  • SUZUKI Yukio
    Institute for Agricultural and Biological Sciences, Okayama University

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Other Title
  • アスコルビン酸糖化合物の酵素的生成(予報)
  • アスコルビン酸糖化合物の酵素的生成-予-
  • アスコルビンサン トウ カゴウブツ ノ コウソテキ セイセイ ヨ

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Description

By paper chromatography, two new spots having reducing power on 2,6-dichlorophenol-indophenol and silver nitrate beside the spot of L-ascorbic acid were found in the incubation mixture containing partially purified transglucosidase from Aspergillus niger IAM 2534,maltose, sodium L-ascorbate and thiourea. The new derivatives were also precipitated as lead compounds in diluted alkali solution like as L-ascorbic acid. The lead precipitate was purified by washing with diluted alkali solution, and by repeated process of liberation from lead precipitate at pH 3.0 by sulfuric acid and reprecipitation as lead compounds in diluted alkali solution until no more free sugar was detected on paper chromatogram. Hydrolysis of the purified derivatives in N H_2SO_4 or with the partially purified transglucosidase from Asp. niger gave D-glucose and L-ascorbic acid. D-Glucose was identified by glucose oxidase and by paper chromatography. From these results, two new derivatives enzymatically formed from AsA and maltose are thought to be L-ascorbic acid glucoside-like and its oligosaccharide-like compounds.

Journal

  • VITAMINS

    VITAMINS 43 (5), 205-209, 1971

    THE VITAMIN SOCIETY OF JAPAN

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